Decoding molecular and cellular heterogeneity of nucleus accumbens with high-throughput scRNA-seq and MERFISH
Decoding molecular and cellular heterogeneity of nucleus accumbens with high-throughput scRNA-seq and MERFISH
Abstract The nucleus accumbens (NAc) plays an important role in regulating multiple behaviors and its dysfunction has been linked to many neural disorders. However, the molecular, cellular and anatomic heterogeneity underlying its functional diversity remains incompletely understood. Here, we generate a cell census of the mouse NAc using high-throughput single cell RNA sequencing and multiplexed error-robust FISH, revealing a high level of cell heterogeneity in this brain region. We show that the transcriptional and spatial diversity of neuron subtypes underlie NAc’s anatomic and functional heterogeneity, and possibly contribute to the pathogenesis of different neurological disorders. These findings explain how the seemingly simple neuronal composition of the NAc achieves its highly heterogenous structure and diverse functions. Collectively, our study generates a spatially resolved cell taxonomy for understanding the NAc structure and function, which demonstrates the importance of combining molecular and spatial information in revealing the fundamental features of the nervous system.
Chen Renchao、Bhattacherjee Aritra、Chen Wenqiang、Tuesta Luis M.、Zhang Yi、Hao Junjie、Zhuang Xiaowei、Djekidel Mohamed N.、Blosser Timothy R.
Howard Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs HospitalHoward Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs HospitalHoward Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs HospitalHoward Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs HospitalHoward Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs Hospital||Department of Genetics, Harvard Medical School||Harvard Stem Cell InstituteHoward Hughes Medical Institute, Harvard University||Department of Chemistry and Chemical Biology, Harvard University||Department of Physics, Harvard UniversityHoward Hughes Medical Institute, Harvard University||Department of Chemistry and Chemical Biology, Harvard University||Department of Physics, Harvard UniversityHoward Hughes Medical Institute, Boston Children?ˉs Hospital||Program in Cellular and Molecular Medicine, Boston Children?ˉs Hospital||Division of Hematology/Oncology, Department of Pediatrics, Boston Children?ˉs HospitalHoward Hughes Medical Institute, Harvard University||Department of Chemistry and Chemical Biology, Harvard University||Department of Physics, Harvard University
神经病学、精神病学基础医学分子生物学
Single-cell RNA-seqMERFISHNucleus accumbensanatomic complexityfunctional diversity
Chen Renchao,Bhattacherjee Aritra,Chen Wenqiang,Tuesta Luis M.,Zhang Yi,Hao Junjie,Zhuang Xiaowei,Djekidel Mohamed N.,Blosser Timothy R..Decoding molecular and cellular heterogeneity of nucleus accumbens with high-throughput scRNA-seq and MERFISH[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/2021.07.17.452808.点此复制
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