OE1介导独脚金内酯调控拟南芥开花的机制研究

s is well known, flowering regulation is one of the important processes in plant growth and development. Some plant hormones, such as gibberellins and auxins, play crucial roles in flowering regulation. Strigolactones (SLs) are a novel class of terpenoid plant hormones derived from β-carotene, which play an important role in regulating plant growth and development. Previous studies have suggested that SLs may play a role in regulating plant flowering, but their exact function and molecular mechanism remain to be further elucidated. In this study, using Arabidopsis as the material, the phenotype identification of SL series mutants confirmed the inhibitory effect of endogenous SL on Arabidopsis flowering. Subsequently, through library screening, the TOE1 factor was identified, which interacts with the SL receptor D14 and the SL signaling repressor SMXL7. Statistical analysis of the flowering phenotypes of a series of multiple mutants and overexpression mutants revealed that TOE1 is a key factor in the SL signaling pathway regulating flowering and acts downstream of SMXL6/7/8. Furthermore, the interaction between SMXL7 and TOE1 inhibits its transcriptional repression activity, while D14 enhances its transcriptional repression activity, thereby respectively activating and inhibiting downstream FT genes. This study elucidates the function and related molecular mechanism of SL in regulating plant flowering time, not only enriching the understanding of SL signal transduction regulatory mechanisms but also laying the foundation for unraveling the plant flowering time regulation network.