检测布氏杆菌抗体直接阻断ELISA方法的建立
evelopment of directly blocking enzyme-linked immunosorbent assay for antibodies against Brucellaellosis
本研究利用研制的抗布鲁氏菌特异性单克隆抗体5C10建立了检测血清中布鲁氏菌抗体的直接阻断ELISA方法，并对该方法反应条件进行了优化，组建了试剂盒。用该试剂盒与进口ELISA试剂盒对已知背景的75份牛血清和25份羊血清样品进行检测比较，结果显示，本研究建立的检测布氏杆菌病血清抗体直接阻断ELISA方法的特异性和敏感性好，且结果稳定，与进口试剂盒检测结果符合率达到100%。该试剂盒将在我国布鲁氏菌病的净化与根除工作中具有重要应用价值。
A directly blocking enzyme-linked immunosorbent assay (ELISA) to detect the antibodies against Brucellaella was developed with monoclonal antibody (mAb) 5C10 to B.abortus. Main factors related to the effect of blocking ELISA reaction were optimized and a kit was made. 75 Bovine sera samples and 25 sheep sera samples were detected by both our kit and commercial kit. Results showed that the detection coincidence rate was 100%. Directly blocking ELISA to test sera against Brucellaella is very sensitivity, specificity and stability. It will be very important useful in the eradication of Brucellosis.
朱桥平、叶建强、宰栩生、吴雯、邵红霞、秦爱建、钱琨、王超凡
医学研究方法微生物学预防医学
布鲁氏菌病单克隆抗体阻断ELISA
BrucellaellosisMonoclonal antibodyBlocking ELISA
朱桥平,叶建强,宰栩生,吴雯,邵红霞,秦爱建,钱琨,王超凡.检测布氏杆菌抗体直接阻断ELISA方法的建立[EB/OL].(2016-09-28)[2025-07-16].http://www.paper.edu.cn/releasepaper/content/201609-253.点此复制
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