蚕蛹中耐热蛋白的发现和分离纯化

thermophilic bacterium protein "Cut A1" was studied by Harima institute in 2006, it was destructed at 148.5℃, it is the most heat-resistant protein currently. In the paper, it adopted a way of "cook" to found that there are existed thermpstabilit protein, sex-specific storage protein 2( SSP2) and serpin. Although the separation and purification of natural proteins is still a nodus, the "cook" step can be removed by the majority of hybrid protein, to obtain crude sample, and then to obtain a high puruty of sample by ammonium sulfate precipitation, SuperdexTM200, Resource Q and so on. In this paper, proved that SSP2 is a hexamer in natural state, and SSP2 have obvious anti-apoptotic effect on BmN cell. At the same time, it is found that serpin is still active after 100℃ treatment. The DSC scan result showed that the denaturation temperature of serpin is up to 165.5℃ in PBS ( pH 7.4 ), it higher 17℃ than currently the world's most thermostabilit protein. The experimental for studying thermostabilit protein structure and silkworm protein function laid the foundation in future, and as a reference for research related protien functions.