表面增强拉曼光谱快速检测硝基呋喃类抗生素呋喃妥因与呋喃它酮

Nitrofurans are a group of widely used veterinary antibiotics which have been forbidden in many countries. Surface-enhanced Raman spectroscopy (SERS) coupled with gold nanoparticles was used in this study for rapid detection and characterization of nitrofuran antibiotics (including furadantin, furaltadone and their mixture). Density functional theory calculations was conducted with Gaussian 03 at the B3LYP level using the 6-31G(d) basis set to analysis the assignment of vibrations. The SERS measurement of nitrofuran antibiotics has been investigated by active SERS substrate of gold nanoparticles with average size around 50 nm. The SERS qualitative analyses has been studied for furadantin and furaltadone by their characteristic SERS pesks. The peaks at 1420 cm-1 and 1456 cm-1 could be used to discriminate their mixture. The SERS peaks at 1008 cm-1 and 1162 cm-1 were used as the index of quantitative analysis for furadantin and furaltadone, respectively. Under the optimal conditions, the limit of detection could reach the level of 5 ppm. The results showed that the SERS method with gold nanoparticles was capable of identifying and characterizing furaltadone and furadantin, which was valuable to be further applied on the detection of antibiotic residues in food sample.