HPD-600型大孔树脂对维生素B12吸附与解吸性能的研究
he Research of Adsorption and Desorptionof Vitamin B12 by HPD_600 Resin
维生素B12(钴胺素)是一类含钴的维生素的总称。它是一种造血维生素。血红细胞的组成、正常神经细胞的活动以及细胞分裂期DNA的合成都需要维生素B12。人体缺乏维生素B12可引起多种疾病,如巨幼细胞贫血、疲劳、食欲不振、心情烦躁等。吸附树脂是一种新的聚合物吸附剂,是利用树脂能发生吸附—解吸这一作用,以达到物质的分离,纯化目的的一类可以反复利用的树脂。它不易受盐,酸,碱等杂质的影响,而且吸附质被树脂吸附后,再从树脂上洗脱而得到的产品纯度很高,可以重复利用。是很好的分离提纯手段。本课题主要采用紫外分光光度法和高效液相色谱分析法,研究HPD_600型吸附树脂对维生素B12的吸附和解吸能力,以及床层体积,液体流动速度等对动态吸附和解吸的影响,为以后能工业化的分离提纯做前期准备工作。
Vitamin B12(cyanocobalamin) is a cobalt-containing B complex vitamin. Cyanocobalamin is a haemopoietic vitamin. It is necessary for the formation of red blood cells, for normal nerve cell activity and for the synthesis of DNA during cell division. Vitamin B12 deficiency causes various diseases, such as macrocytic anemia, fatigue, loss of appetite, and mood disturbances.Adsorption resin is a new absorbent polymer. It cannot be influence for such as salt, acid and alkali. After the absorption of the resin adsorption, from the resin, the high purity of products can be obtains, It can be used repeat. It is a good means of separation and purificationUV-spectrophotometry and High-Performance Liquid Chromatography is used to studies the ability of adsorption and desorption of CAD-45 resin to Vitamin B12b, and the influence of Bed level volume, fluid velocity and so on to dynamic adsorption and desorption in this research. Prepare for the industrialization of purification for the future.
诸葛倩、邱烨
制药化学工业生物科学研究方法、生物科学研究技术化学工业概论
钴胺素吸附树脂紫外分光光度法高效液相色谱
Hydroxocobalamindsorption resin UV-spectrophotometryHigh-Performance Liquidhromatography
诸葛倩,邱烨.HPD-600型大孔树脂对维生素B12吸附与解吸性能的研究[EB/OL].(2009-09-01)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/200909-16.点此复制
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