PLATELET BINDING, ELECTROPHORESIS AND BIOASSAY BASED IDENTIFICATION OF THE BAOACTIVE COMPONENT IN SNAKE VENOM

rude Venom of Agkistrodon halys palls (f0) were separated by use of sepadex-100 column and 6 fractions (f1-6) were obtained. In the bioassay, for instance anti-thrombosis assay, anti-aggregation assay, mice bleeding time test, bovine fibrin plate assay of the main fractions (f1-4), f4 exhibited the highest potency in the anti-thrombosis related experiments and f3 exhibited the highest potency in the thrombolysis related experiments. The results from the fixed platelet binding experiment and the electrophoresis indicated that there were two strips (approximate molecular weight 20000 and 28000) in f4 should be specifically responsible for its excellent anti-thrombosis related activities. The results from the euglobulin clot lysis experiment and the electrophoresis indicated that there were also two strips (approximate molecular weight 48000 and 52000) in f3 should be specifically responsible for its good thrombolysis related activities.