酸法脱酰胺结合酶解制备米蛋白肽及其与亚铁螯合工艺的研究

In this paper, rice protein with acid deamidation was used as the raw material to prepare peptides by enzymatic hydrolysis. The preparation of the rice protein peptide-iron complex and the iron-chelating capacity of this complex were then investigated. Alcalase 2.4 L was the best protease and revealed a much higher potential to hydrolyze rice protein.The degree of hydrolysis and the yield of protein peptides after 4 hours' enzymolysis reached 23.4% and 23.4%, respectively.The yield of protein peptides was effectively improved after acid deamidation. The acid deamidation conditions were: 0.4 mol/l HCl, temperature at 95 ℃ and time 3 h. Single factor experiment showed that temperature, pH and mass ratio of hydrolysis peptides and ferrite had effects on the yield and iron-chelating capacity of the peptide-iron complex. The optimal conditions for enzymatic hydrolysis peptides to chelate iron were showed as follows: temperature at 50 ℃, mass ratio in 2:1, pH at 6.5. Under these conditions, the yield of this complex was 65.2% and the maximum level of iron-chelating capacity was 21.6 mg/g.