川芎嗪对大鼠缺氧性呼吸抑制的保护作用
Protective effect of tetramethylpyrazine on the respiratory inhibition induced by hypoxia in rats
探讨川芎嗪（tetramethlpyrazine, TMP）对缺氧大鼠呼吸中枢的保护作用。 制备缺氧大鼠模型，观察TMP对缺氧大鼠的呼吸活动和存活时间的影响，采用尼氏染色和免疫组化方法分别检测TMP对缺氧大鼠脑干神经元尼氏体及FOS蛋白表达的影响。 缺氧可引起动物呼吸先兴奋后抑制，最后导致动物死亡，TMP可显著推迟缺氧性呼吸抑制和动物死亡的发生（P<0.01）；与空气对照组相比，缺氧后脑干神经元尼氏染色着色变浅，但缺氧加TMP组着色深于单纯缺氧组，其灰度值明显低于单纯缺氧组（P<0.05）；缺氧后FOS蛋白在脑干的阳性表达神经元主要见于孤束核、最后区、舌下神经核、中缝苍白核、下橄榄核、外侧网状核、面神经核、斜方体核，但缺氧加TMP组的阳性表达强度明显低于单纯缺氧组（P<0.05）。 TMP对呼吸中枢的缺氧性损害具有保护作用，脑干内FOS蛋白可能参与了这一过程。
o study the protective effect of tetramethylpyrazine (TMP) on the respiratory inhibition induced by hypoxia. The hypoxic rat model was established. The effect of TMP on the responses of respiratory activity and life time to hypoxia were observed. The Nissil staining and immunohistochemical technique were used to investigate the effect of hypoxia on the Nissil bodies and FOS expression in the brainstem and the antagnistic action of TMP. A successive process of response, respiratory excitation followed by inhibition and death in the end of the animals, was induced by hypoxia, but in the rats pretreated with TMP, the occurence of respiratory inhibition was delayed and the life time was prolonged obviously (P<0.01). Compared with the air contronl group, the intensity of the Nissil staining of the brainstem neurons became weaker in the group of hypoxia, but in the group of hypoxia plus TMP, the Nissil staining was stronger and the gray degree decreased obviously compared with the group of
呼海燕、李丽、郑煜、杨文星、李自成、陈丽、严亨秀
基础医学生理学药学
川芎嗪 缺氧 呼吸 FOS 大鼠
etramethylpyrazine Hypoxia Respiration FOS Rat
呼海燕,李丽,郑煜,杨文星,李自成,陈丽,严亨秀.川芎嗪对大鼠缺氧性呼吸抑制的保护作用[EB/OL].(2005-01-13)[2025-08-23].http://www.paper.edu.cn/releasepaper/content/200501-50.点此复制
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