菊花DgCCD7启动子克隆与顺式作用元件分析

Strigolactone is known as a long-distance signal in regulation of chrysanthemum shoot branching. The promoter of SL biosynthesis gene, DgCCD7, was isolated from chrysanthemum (Dendranthema grandiflorum cv. Jinba), which contains full-length of 1442bp. By using PLACE database, analysis of promoter sequence of DgCCD7 was carried out. Associated with DgCCD8 promoter, common motifs were identified. Two auxin responsive motifs and one PIBs binding motif were identified on both DgCCD7 and DgCCD8 promoters. By transformation of Arabidopsis fusion with GUS gene, DgCCD7 promoter can be activated at meristems and hypocotyledonary region. Meanwhile NAA and Pi starvation can active GUS expression in roots of transgene Arabidopsis. To sum up, this study can pave the great way for regulation mechanism research in chrysanthemum shoot branching, which can be used for controlling branching during cut flower production..