多年生黑麦草LpMAX2的鉴定及功能研究

Strigolactone (SL) is a novel plant hormone that plays an important role in regulating plant growth and development. The F-box protein MAX2 is a key element in the SL signaling pathway and participates in most of the growth and development processes regulated by SL. Perennial ryegrass (Lolium perenne L.), belonging to the genus Ryegrass of Gramineae, is an important turfgrass and forage grass, and its branching and plant height are important traits. In this study, to explore the regulatory function of perennial ryegrass LpMAX2 on the height of its branches, we successfully cloned the LpMAX2 gene based on the genomic information of ryegrass. The homozygous 35S:LpMAX2/max2-3 line was obtained by infecting the Arabidopsis max2-3. Moreover, we observed the phenotypes of Col-0 and LpMAX2, and found that LpMAX2 could partially rescue the phenotypic defects of max2-3, indicating that LpMAX2 had a certain degree of conservation in regulating plant branching and plant height, although it did not fully recover to the level of wild type Col-0. In addition, the genetic transformation system of perennial ryegrass was established, and the transgenic positive regenerated plants were successfully obtained, which laid a foundation for further research on the function of LpMAX2.