LC-3基因在宫颈癌中的表达及临床意义

Objectives To study the expression of CLC-3 mRNA in cervical cancer. Methods Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of CLC-3 mRNA in 60 patients with cervical cancer,10 patients with cervical cancer before and after radiotherapy and chemotherapy, and 10 patients with normal cervical tissue. Results the ratio of gray level of CLC-3 mRNA was 0.6912 in normal cervical tissue, 2.3937 in cervical cancer, there were significant differences between them (P<0.01). The ratio of gray level of CLC-3mRNA was 4.5899 in the poor differentiation cancer, higher than that of the middle and high differentiation cancer 1.5718 and 1.3187 (P<0.05). the ratio of gray level of CLC-3mRNA was 1.0842 in stages Ⅰ～Ⅱ, 3.5688 in stages Ⅲ～Ⅳ, there were significant differences between them (P<0.01). CLC-3mRNA was 1.5198 before radiotherapy and chemotherapy, but 0.2237 after radiotherapy and chemotherapy, they have significant differences(P<0.01). Conclusion CLC-3mRNA participates in the proliferation of cervical cancer cell, and plays an important role in tumorgenesis of cervical cancer. CLC-3mRNA have a positive correlation with clinical stages and pathological grades of cervical cancer. The expression of CLC-3mRNA in cervical cancer was decreased after radiotherapy and chemotherapy.