Survey of SARS-CoV-2 genetic diversity in two major Brazilian cities using a fast and affordable Sanger sequencing strategy
Survey of SARS-CoV-2 genetic diversity in two major Brazilian cities using a fast and affordable Sanger sequencing strategy
Abstract Genetic variants of SARS-CoV-2 have been emerging and circulating in many places across the world. Rapid detection of these variants is essential since their dissemination can impact transmission rates, diagnostic procedures, disease severity, response to vaccines or patient management. Sanger sequencing has been used as the preferred approach for variant detection among circulating human immunodeficiency and measles virus genotypes. Using primers to amplify a fragment of the SARS-CoV-2 genome encoding part of the Spike protein, we showed that Sanger sequencing allowed us to rapidly detect the introduction and spread of three distinct SARS-CoV-2 variants in two major Brazilian cities. In both cities, after the predominance of variants closely related to the virus first identified in China, the emergence of the P.2 variant was quickly followed by the identification of the P1 variant, which became dominant in less than one month after it was first detected.
Durigon Edison Luiz、Dorlass Erick Gustavo、Louren?o Karine Lima、Fiorini Alex、Coelho Helena Perez、Peixoto Renata、Sato Hugo、Leal Oliveira Danielle Bruna、Ribeiro Teixeira Santuza Maria、Thomazelli Luciano Matsumiya、Miserani Magalh?es Rubens Daniel、Telezynski Bruna Larotonda、Scagion Guilherme Pereira、Fernandes Ana Paula、Guimar?es Fonseca Flavio、Ometto Tatiana
Universidade de S?o PauloUniversidade de S?o PauloCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteHospital Israelita Albert EinsteinCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteUniversidade de S?o PauloCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteUniversidade de S?o PauloUniversidade de S?o PauloCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteCentro de Tecnologia de Vacinas, Universidade Federal de Minas Gerais, Belo HorizonteUniversidade de S?o Paulo
医学研究方法遗传学分子生物学
Sars-Cov-2genetic variantsSpikeSanger Sequencing
Durigon Edison Luiz,Dorlass Erick Gustavo,Louren?o Karine Lima,Fiorini Alex,Coelho Helena Perez,Peixoto Renata,Sato Hugo,Leal Oliveira Danielle Bruna,Ribeiro Teixeira Santuza Maria,Thomazelli Luciano Matsumiya,Miserani Magalh?es Rubens Daniel,Telezynski Bruna Larotonda,Scagion Guilherme Pereira,Fernandes Ana Paula,Guimar?es Fonseca Flavio,Ometto Tatiana.Survey of SARS-CoV-2 genetic diversity in two major Brazilian cities using a fast and affordable Sanger sequencing strategy[EB/OL].(2025-03-28)[2025-06-06].https://www.medrxiv.org/content/10.1101/2021.07.02.21259802.点此复制
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