Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice
Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice
Abstract Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre-recombinase-dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or circadian locomotor activity rhythms. In vivo and ex vivo studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei ex vivo. In vivo studies show Dbp-driven bioluminescence rhythms in the liver of Albumin-Cre;DbpKI/+ “liver reporter” mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food ad libitum. These studies confirm the previously demonstrated circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.
Garbutt Lauren A.、Martin-Burgos Blanca、van der Vinne Vincent、Stowie Adam C.、Molyneux Penny C.、Harrington Mary E.、Weaver David R.、Smith Ciearra B.、Dallmann Robert、Davidson Alec J.、McCartney Eleanor、Brodsky Michael H.、Leise Tanya L.
Division of Biomedical Sciences, Warwick Medical School, University of WarwickNeuroscience Program, Smith CollegeDepartment of Neurobiology, University of Massachusetts Chan Medical School||Department of Biology, Williams CollegeNeuroscience Institute, Morehouse School of MedicineNeuroscience Program, Smith CollegeNeuroscience Program, Smith CollegeDepartment of Neurobiology, University of Massachusetts Chan Medical School||Graduate Program in Neuroscience, University of Massachusetts Chan Medical School||NeuroNexus Institute, University of Massachusetts Chan Medical SchoolDepartment of Neurobiology, University of Massachusetts Chan Medical School||Graduate Program in Neuroscience, University of Massachusetts Chan Medical SchoolDivision of Biomedical Sciences, Warwick Medical School, University of WarwickNeuroscience Institute, Morehouse School of MedicineNeuroscience Program, Smith CollegeDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Chan Medical SchoolDepartment of Mathematics and Statistics, Amherst College
细胞生物学生理学分子生物学
Circadian RhythmsBioluminescenceLuciferaseMisalignmentDbpAlbumin D-element binding proteinIn Vivo Imaging System (IVIS)LumiCycle In VivoReporter MousePeripheral Oscillators
Garbutt Lauren A.,Martin-Burgos Blanca,van der Vinne Vincent,Stowie Adam C.,Molyneux Penny C.,Harrington Mary E.,Weaver David R.,Smith Ciearra B.,Dallmann Robert,Davidson Alec J.,McCartney Eleanor,Brodsky Michael H.,Leise Tanya L..Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2021.04.04.438413.点此复制
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