双分子荧光互补技术研究进展

In the past decade, the bimolecular fluorescence complementary (BiFC) has become an important means of study protein interactions。It has been proved very useful in cultured cells and a variety of model organisms. The BiFC assay is based on reconstitution of an intact fluorescent protein when two complementary non-fluorescent fragments are brought together by a pair of interacting proteins. Here, we look back at the history of the development of the BiFC technology, reviewed on the development of the BiFC system, and possible improvements in the future from a technical point of view.