FLII蛋白抑制肺腺癌细胞H1299的迁移和侵袭

H1299 is a highly invasive lung adenocarcinoma cell. FLII protein has been shown by literatures to be involved in cytoskeletal rearrangement and cell migration and invasion. However, its role in the migration and invasion of H1299 cells has not yet been reported. In this study, we firstly detected the expression level of FLII protein in normal cells, lung cancer cells, and breast cancer cells. Our results indicated that FLII expression in H1299 and A549 lung cancer cells was significantly lower than that in the control HBE cells. We have constructed the eukaryotic expression vector pCMV-N-Flag-FLII, and transfected it in H1299 cells. Then, By screening using G418, we established the H1299 cell line stably expressing Flag-FLII. Transwell experiments using these cells demonstrated that FLII could inhibit the migration and invasion of H1299 cells, which provides a useful tool for further study on the biological function of FLII protein.