银杏叶黄素皂化工艺研究

Lutein, which is widely present in vegetables, flowers and fruit and other plants, is a kind of important natural antioxidant. In this paper, the extract of petroleum ether from Ginkgo biloba leaf were used as raw material, the influence of the saponification time, hydrolysis temperature and ratio of material to liquid on the yield of Ginkgo lutein were investigated. On the basis of single factor experiment, the Box-Behnken test was designed.It was indicated that the optimum saponification conditions: the saponification time 2.5 h, saponification temperature 58 ℃, solid-liquid ratio was 3∶1, the lutein yield reached 1.19 mg/ml. Analyzed by HPLC, the saponification of Ginkgolutein purity reached 90.1%.