SPOI标记猪脂肪干细胞向成骨细胞分化及体外MRI成像

OBJECTIVE: To study the effects of superparamagnetic iron oxide nanoparticles (SPIO) labeling on the differentiation potential of adipose tissue-derived stem cells (ADSCs) to osteoblasts, and the characteristics of magnetic labled ADSCs in vitro 3.0T magnetic resonance imaging(MRI). METHODS: ADSCs were isolated from the subcutaneous adipose tissue of experimental pigs and expanded in culture medium. ADSCs were incubated with the SPIO-PLL complex. The differentiation capacity of ADSCs to osteogenic lineage between labeled and unlabeled cells was compared. Different concentrations of labeled ADSCs and the control were scanned at a 3.0T MR system with T1WI , T2WI and T2*WI. RESULTS: Numerous intracytoplasmic iron particles stained with Prussian blue were detected in labeled cells with labeling efficiency of nearly 100%. Labled ADSCs were capable of differentiating to osteoblasts. Both labeled and unlabeled cells were able to differentiate into osteoblasts and calcification formation was found in both osteogenic lineage. The change in MR signal intensity increased at T1WI, T2WI and T2*WI along with elevation of the labeled cells concentration. The marked change in signal intensity were observed at T2*WI . The lowest concentration of SPIO labeled ADSCs detected by 3.0T MRI was 1×106/L cell. CONCLUSION: ADSCs are promising cells in bony tissue engineering and bony grafting.SPIO labled ADSCs could be traced by 3.0T MR.