S-层蛋白晶体生长研究
Research on the Growth of S-layer Protein Crystal
古细菌细胞壁表面的S-层蛋白具有独特的自组装特性，能够在界面上重新自组装形成纳米级二维网格结构，近年来已经成为纳米领域的研究热点。而深入研究S-层蛋白的分子结构将有助于揭示其自组装机理，扩大S-层蛋白自组装结构的应用领域。制备S-层蛋白有序结晶是研究其分子结构研究的重要基础，本文从芽孢杆菌中提取S-层蛋白并观察了其自组装。此外，成功在硅烷化的玻璃表面构建了生物素-亲和素（biotin-avidin）分子链接，促进S-层蛋白在固基表面的自组装与结晶，成功得到了S-层蛋白的规则晶体，为S-层蛋白的三维结构分析提供了研究基础，推动了S-层蛋白自组装及改性机理研究的进展。
S-layer protein has the unique ability to re-assemble into two-dimensional monolayer grid structure and in recent years it has attracted great attention in the field of biomimetic and nanotechnology. The ordered crystalline S-layer protein is an important foundation to study the structure of the protein molecular, we successfully obtained S-layer protein crystal using the silanized glass surface modified by biotin-avdin. These nice protein crystals are expected to contribute to the three-dimensional structure analysis of the protein and promote the progress of the research on self-assembly and modification of S- layer protein.
乐悦、史勤超
生物科学研究方法、生物科学研究技术生物化学分子生物学
S-层蛋白微纳制造晶体结构分析
S-layer ProteinNanotechnologyCrystalStructure Analysis
乐悦,史勤超.S-层蛋白晶体生长研究[EB/OL].(2016-04-25)[2025-08-19].http://www.paper.edu.cn/releasepaper/content/201604-300.点此复制
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