纤维强度不同的品种在棉纤维发育过程中基因表达差异的分析

In this experiment,we employed cDNA-AFLP for studying genes expression differences among phases for rapid elongation, overlapping and secondary wall rapid thickening of fiber by comparing five cotton(Gossypium hirsutum L.) cultivares of high-strength fibers (2001,7235,Beiersinuo, Acala1517-77, PD5264) with one cultivar of nomal fiber(shiyuan321, meifuchangrong ) and one cultivar of high- strength and thin fibers (hai7124)(Gossypium barbadense L.). we accomplished the analysis of gene expression difference covering 256 pairs of selective primers ,and got 271 ESTs. Sixteen fragments were cloned and sequenced，by BlastX in NCBI and fonud three of them is 84% homologous with cellulose synthase mRNA in cotton fiber; 98% homologous with fiber protein Fb8-like mRNA; 99% homologous with lipid transfer protein（LTP）mRNA; the rest are unknown proteins or lack of homologous, we speculate that they are new gene fragments in development of cotton fiber.