酶法合成肝靶向性脂质体载体胆固醇-半乳糖苷工艺研究
he enzymatic synthesis of Cholesterol-vinyl sebacate-lactitol denominated liver-targeting liposomes carrier
目的：利用脂肪酶催化合成肝靶向性脂质体载体胆固醇-半乳糖苷（CH-VS-LA），对其反应条件进行优化。方法：采用质谱和碳谱鉴别产物结构；单因素和正交设计法优化酶法合成条件。结果：酶法最佳条件为底物摩尔比胆固醇癸二酸乙烯酯:乳糖醇 4:1，脂肪酶Novozym435 25mg，反应32h， 产率高达 92%。结论：单因素和正交设计法优化脂肪酶Novozym 435合成脂质体载体是有效可行的。
objective: To synthesize liposomes vector, cholesterol-vinyl sebacate-lactitol (CH-VS-LA) by using enzymatic reaction and to optimize its synthesis process. Methods: MS and 13C-NMR were used to idendify the structure of product, enzymatic synthesis conditions were optimized via single-factor test and orthogonal experimental design. Results: The enzymatic optimum conditions are the followings: the molar ratio of CH-VS to LA 4:1, the amount of lipase Novozym435 25mg, reactional time 32h, productive rate 92%. Conclusion: The method that lipase Novozym435 catalyszes synthesic process of cholesterol-vinyl sebacate-lactitol by single-factor test and orthogonal experimental design is effective and feasible.
郑品劲、陈宇潮、仝一丹、陈静、聂华、程怡、李朝、罗利华
药学生物科学研究方法、生物科学研究技术
酶法合成肝靶向胆固醇-半乳糖苷单因素设计正交设计试验
Enzymatic synthesisliver-targetingcholesterol-vinyl sebacate-lactitolsingle-factor testorthogonal experimental design
郑品劲,陈宇潮,仝一丹,陈静,聂华,程怡,李朝,罗利华.酶法合成肝靶向性脂质体载体胆固醇-半乳糖苷工艺研究[EB/OL].(2015-03-19)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201503-205.点此复制
评论