Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury
Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury
Abstract Stroke is a highly disabling disease with few therapeutic options. Transplanting stem cells into the central nervous system (CNS) is a promising potential strategy. However, preclinical trials of cell-based therapies are limited by poor local cell engraftment and survival. Synthetic scaffolds offer an alternative to optimize stem cell transplantation at sites of brain injury. Here, we present a rotary jet spun polylactic acid (PLA) polymer used as a scaffold to support delivery of mesenchymal stem cells (MSCs) in a mouse model of stroke. We isolated bone marrow MSCs from adult C57/Bl6 mice, cultured them on PLA polymeric rough microfibrous (PLA-PRM) scaffolds obtained by rotary jet spinning, and transplanted into the brains of adult C57/Bl6 mice, carrying thermocoagulation-induced cortical stroke. The expression levels of interleukins (IL4, IL6 and IL10) and tumor necrosis factor alfa (TNFα) in the brain of mice that received PRM were similar to untreated mice. Transplantation of MSCs isolated or cultured on PRM significantly reduced the area of the lesion and PRM delivery increased MSCs retention at the injury site. We conclude that PLA-PRM scaffolds offer a promising new system to deliver stem cells to injured areas of the CNS.
Reis Marcella B.C.、Galindo Layla T.、Lobo Anderson O.、Mundim Mayara T.V.V.、Marciano Fernanda R.、Zamproni Laura N.、Grinet Marco A.V.M.、Porcionatto Marimelia
Neurobiology Lab, Universidade Federal de S?o Paulo||Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de S?o PauloNeurobiology Lab, Universidade Federal de S?o Paulo||Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de S?o PauloLaboratory of Biomedical Nanotechnology, Universidade Brasil||Department of Medicine, Brigham and Women?ˉs Hospital||Nanomedicine Lab, Department of Chemical Engineering, Northeastern University||Interdisciplinary Laboratory for Advanced Materials, PPGCM, Technology Center, Universidade Federal do PiauiNeurobiology Lab, Universidade Federal de S?o Paulo||Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de S?o PauloLaboratory of Biomedical Nanotechnology, Universidade Brasil||Department of Medicine, Brigham and Women?ˉs Hospital||Nanomedicine Lab, Department of Chemical Engineering, Northeastern UniversityNeurobiology Lab, Universidade Federal de S?o Paulo||Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de S?o PauloInstituto Tecnol¨?gico da Aeron¨￠utica, Pra?a Marechal Eduardo GomesNeurobiology Lab, Universidade Federal de S?o Paulo||Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de S?o Paulo
神经病学、精神病学基础医学生物科学研究方法、生物科学研究技术
mesenchymal stem cellspolymeric rough microfibersrotary-jet spinningPLAbrain injurystroke
Reis Marcella B.C.,Galindo Layla T.,Lobo Anderson O.,Mundim Mayara T.V.V.,Marciano Fernanda R.,Zamproni Laura N.,Grinet Marco A.V.M.,Porcionatto Marimelia.Rotary jet-spun porous microfibers as scaffolds for stem cells delivery to central nervous system injury[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/239194.点此复制
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