夹心ELISA检测牛γ-干扰素方法的建立及应用

o establish a sandwich ELISA for detection bovine interferon γ (BoIFN-γ), three monoclonal antibodies against BoIFN-γ were coated as capture antibody to detect natural BoIFN-γ. The result showed that all of the three monoclonal antibodies could recognize BoIFN-γ. After the exploration and optimization, the sandwich ELISA for detecting BoIFN-γ were established by 3E1 as capture antibody and HRP conjugated 6E5 as detect antibody respectively. For the evaluation of this sandwich ELISA, 66 serum samples from different regions were assessed both by the sandwich ELISA and by the commercial ELISA kit. The agreement between these two tests was 95.45%, which revealed that this sandwich ELISA method could effectively detect interferon γ in bovine tuberculosis disease.