桑树亚硝酸还原酶基因的克隆及生物信息学分析

NiR primer was designed According to Morus notabilis genome database Scafford Morus02756. Guiyou62 cDNA was used as PCR template to get the full length .The full length of cDNA was 1764bp, encoding 587 amino acids . Sequencing analysis showed that it was 76％ to88％ identical with the sequences of other reported plants. the gene sequence is submitted to NCBI for login, the accession number is: Gen Bank: KC823634.we name as MaNiR.Using Muscle software to conduct the multiple comparison of NiR amino acid sequence and constructing the phylogenetic tree to conduct N-J clustering analysis. Nitrite reductase gene encoding amino acid sequences into three, respectively, algae, monocoty-ledon and dicotyledon.The genetic relationship between MaNiR and perennial fruit tree birch, citrus, prunus persica and strawberry is very close.