急性髓系白血病CDX2和β-catenin基因表达的相关性

Objective To investigate the expressions of CDX2 and -catenin and their correlation in acute myeloid leukemia (AML). Methods Real-time PCR was used to examine the expressions of CDX2 and -catenin mRNA in 92 de novo AML and 30 non-malignant patients (control), and Western blot was employed to detect CDX2 and -catenin protein expressions in 26 de novo AML and 8 non-malignant patients. Results The positivity rates of CDX2 mRNA and protein expressions in AML group were 84.78% and 76.92%, respectively, but no CDX2 mRNA or protein expression were detected in control group (P< 0.01). The expressions of -catenin mRNA and protein were detected in all the patients in both groups but its expressions in AML group were significantly higher (P<0.01). The expressions of CDX2 and -catenin mRNA were significantly correlated with WBC counts and LDH levels (P<0.01). The expression of CDX2 mRNA was not detected and the expression of -catenin mRNA was decreased to normal level in AML patients with complete remission, while the expressions of CDX2 and -catenin mRNA were increased again in those with disease relapse. There were significantly positive correlations between CDX2 and -catenin expressions at both mRNA and protein levels (P<0.01). Conclusion Up-regulation of CDX2 gene and activation of Wnt/-catenin pathway co-exist in AML patients and the expressions of CDX2 and -catenin are positively correlated.