远志挥发油成分提取工艺及GC-MS分析

Objective: To study the best extraction method of volatile oil from polygala tenuifolia, and analyze the chemical constituents, which provided a reference for evaluating its medicinal value. Methods: Water steam distillation method was applied to extract volatile oil, the amount of wate, soaking time, distillation time and ultrasonic time was optimizd orthogonal test. Chemical components in volatile oil were analyzed by GC-MS, the relative content of each component was determined by peak area normalization method. Results: The optimal water-extraction process was as follow: crushing degree was 10 mesh, the water amount was 7 times of the traditional Chinese drugs, no soaking time, the extraction time was 5 hours. 16 compounds were identified and their relative contents were determined. Conclusion: The total contents of 16 compounds identified from volatile oil of polygala tenuifolia was about 100%, among them Cis-Vaccenic acid was the major chemical constituents in the volatile oil which accounting for 62.67%, following was the n-Hexadecanoic acid which accounting for 17.43%. Meanwhile, a scientific basis was provided for determination the extraction process of volatile oil of polygala tenuifolia.