钙网蛋白与OVA融合蛋白的纯化及其生物学功能

o express and purify the fusion protein of calreticulin(CRT) with a fragment of chicken ovalbumin(OVA) and to make a preliminary study on its biological function. Now the vector containing OVA161-276-CRT39-272 was constructed by overlap PCR. The expression of OVA161-276-CRT39-272 fusion protein was induced in genetic engincering strain. The protein was purified from souble fraction of bacteria by His-Trap metal chelation chromatography. The bio-activity of this fusion protein was also investigated. As a result, the vector is successfully constructed and OVA161-276-CRT39-272 has a purity over 95%. The fusion protein can promate the maturation of dendritic cells and stimulate it producte more cytokines than the control protein. OVA161-276-CRT39-272 indeed has some biological function and the result lay down a certain foundation for future research.