55-FU 致小鼠肠黏膜严重损伤时β-catenin 的表达及其意义

Objective To discusse the expression of -catenin proten and its significance in mouse intestinal mucosa, and-catenin was colse related to the proliferation of intestinal stem cells. Methods A total of 70 C57/BL mice were divided twogoups randomly, 30 of them were in control group (group A), and other 40 mice were in experimental group. The mice inexperimental group were treated with introperitoneal injection of large dose of 5-FU (150 mg/kg body weight for 5 consecutivedays), the 30 mice were divied 3 groups, 10 of them were kill at the 1st the 3rd day and the 5th day after the injection wasfinished. The middle of intestine from these mice was fixed for HE straining and immunohistochemical techniques. Theexperession of -catenin in the intestinal sample was detected by immunohistochemical techniques.The mice in control groupwere introperitoneal injected saline for five days as control, and them were treated as the mice in experimental group. ResultsAfter injectin hight dose of 5-FU, the intestine of the mice suffered severe damage: the mucosa was shown as inflammatoryoedema, the villi and cryptes disappared, and the mucosa repaired minimally with time. The expression of -catenin proten inintestianl mucosa of the mice after injecting hight dose of 5-FU increased significantly comparied with control the mice incontrol group, and percentage of -catenin proten positive cells in intestinal mucosa gradually decreased with time in mice ofexperimental group. Conclusion The expression of -catenin in intestinal mucosa increased when the mice were injected hightdose of 5-FU, meaning the proten could promote the proliferation of intestinal stem cells and finish the regeneration ofintestinal mucosa.