高效液相色谱拆分两种β-受体阻断剂药物

Solid-phase extraction (SPE) pre-treatment method and HPLC fluorescence detection method were established to detect aqueous solution of two β-receptor blocker drugs (propranolol and metoprolol). Using two HLB SPE columns (from Oasis and Supel company) for sample pre-treatment，the recovery rates of propranolol and metoprolol in aqueous solution reached 97.33% and 96.08%, respectively. The enantiomeric resolution of propranolol and metoprolol was studied using a teicoplanin based chiral stationary phase colume, with methanol: acetic acid: triethylamine (100: 0.01: 0.01, v/v/v) used as mobile phase at a flow rate of 0.8 mL/min at 20℃. Chiral isomers of two β-blocker drugs can be separated effectively. This method is accurate, simple and reliable, which can be used for propranolol and metoprolol enantiomer detection in water samples.