中间锦鸡儿CiMYB68基因克隆及表达分析

s one of the largest plant transcription factor families, MYB transcription factors play important roles in plant stress tolerance. Here, a MYB68 encoding gene was cloned by rapid amplification of cDNA end technique from Caragana intermedia. The gDNA and full-length cDNA sequence analysis revealed that this gene contains no intron. The full length ORF was 854bp, and the protein deduced comprised 284 amino acids with a calculated molecular weight of 31459.4Da, as well as an isoelectric point of 8.95. The sequence alignment showed that this MYB protein is relatively close to GmMYB68, with an identity of 67%, so the gene was named as CiMYB68. Real-time quantitative PCR analysis showed that the transcript of CiMYB68 was induced strongly under drought and cold treatment. These results indicated that CiMYB68 might be involved in stress responses of C. intermedia.