Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants
Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants
Abstract Multiple plant lineages have independently evolved sex chromosomes and variable karyotypes to maintain their sessile lifestyles through constant biological innovation. Morus notabilis, a dioecious mulberry species, has the fewest chromosomes among Morus spp., but the genetic basis of sex determination and karyotype evolution in this species have not been identified. Three high-quality genome assemblies generated of Morus spp. (including those of dioecious M. notabilis and Morus yunnanensis) were within the range 301-329 Mb in size which were grouped into six pseudochromosomes. Using a combination of genomic approaches, we showed that the putative ancestral karyotype of Morus was close to 14 protochromosomes, and that several chromosome fusion events resulted in descending dysploidy (2n = 2x = 12). We also characterized a ～6.2-Mb sex-determining region on chromosome 3. The four potential male-specific genes, including a partially duplicated DNA helicase gene orthologue (named MSDH) and three Ty3_Gypsy long terminal repeat retrotransposons (named MSTG), were solely identified in the Y-linked area and considered to be strong candidate genes for sex determination or differentiation. Population genomic analysis showed that Guangdong accessions in China were genetically similar to Japanese accessions of mulberry. In addition, genomic areas containing selective sweeps that distinguish domesticated mulberry trees from wild populations in terms of flowering and disease resistance were identified. Our findings provide an important genetic resource for sex identification and molecular breeding in mulberry.
Zhou Yuping、Li Liang、Xiang Zhonghuai、Fan Wei、Zhang Meirong、Bian Peipei、Zhu Baozhong、Wang Xiling、Yu Maode、Zhao Aichun、Liu Shuman、Xia Zhongqiang、Li Zhengang、Jiang Yu、Dai Xuelei、Liu Changying
State Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityCollege of Sericulture, Textile and Biomass Sciences, Southwest UniversityCollege of Sericulture, Textile and Biomass Sciences, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityState Key Laboratory of Silkworm Genome Biology, Institute of Sericulture and Systems Biology, Southwest UniversityThe Sericultural and Apicultural Research Institute, Yunnan Academy of Agricultural SciencesKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, Chengdu University
遗传学植物学分子生物学
MulberryKaryotype evolutionDioecySex determinationPopulation genomics
Zhou Yuping,Li Liang,Xiang Zhonghuai,Fan Wei,Zhang Meirong,Bian Peipei,Zhu Baozhong,Wang Xiling,Yu Maode,Zhao Aichun,Liu Shuman,Xia Zhongqiang,Li Zhengang,Jiang Yu,Dai Xuelei,Liu Changying.Chromosome-level Genomes Reveal the Genetic Basis of Descending Dysploidy and Sex Determination in Morus Plants[EB/OL].(2025-03-28)[2025-06-05].https://www.biorxiv.org/content/10.1101/2022.05.03.490406.点此复制
评论