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Integrated single-nucleotide and structural variation signatures of DNA-repair deficient human cancers

Integrated single-nucleotide and structural variation signatures of DNA-repair deficient human cancers

来源:bioRxiv_logobioRxiv
英文摘要

Mutation signatures in cancer genomes reflect endogenous and exogenous mutational processes, offering insights into tumour etiology, features for prognostic and biologic stratification and vulnerabilities to be exploited therapeutically. We present a novel machine learning formalism for improved signature inference, based on multi-modal correlated topic models (MMCTM) which can at once infer signatures from both single nucleotide and structural variation counts derived from cancer genome sequencing data. We exemplify the utility of our approach on two hormone driven, DNA repair deficient cancers: breast and ovary (n=755 cases total). Our results illuminate a new age-associated structural variation signature in breast cancer, and an independently identified substructure within homologous recombination deficient (HRD) tumours in breast and ovarian cancer. Together, our study emphasizes the importance of integrating multiple mutation modes for signature discovery and patient stratification, with biological and clinical implications for DNA repair deficient cancers.

Boutros Paul C.、Shah Sohrab P.、Grewal Diljot、McKinney Steven、Wang Yi Kan、Zhang Allen、Lesurf Robert、Bashashati Ali、Funnell Tyler、Shiah Yu-Jia

Informatics & Biocomputing, Ontario Institute for Cancer ResearchDepartment of Molecular Oncology, BC Cancer Agency||Department of Pathology and Laboratory Medicine, University of British ColumbiaDepartment of Molecular Oncology, BC Cancer AgencyDepartment of Molecular Oncology, BC Cancer AgencyDepartment of Molecular Oncology, BC Cancer AgencyDepartment of Molecular Oncology, BC Cancer AgencyInformatics & Biocomputing, Ontario Institute for Cancer ResearchDepartment of Molecular Oncology, BC Cancer AgencyDepartment of Molecular Oncology, BC Cancer AgencyInformatics & Biocomputing, Ontario Institute for Cancer Research

10.1101/267500

肿瘤学生物科学研究方法、生物科学研究技术分子生物学

mutation signaturesstructural variantssingle nucleotide variantscancer genomeovarian cancerbreast cancerstatistical modelcorrelated topic models

Boutros Paul C.,Shah Sohrab P.,Grewal Diljot,McKinney Steven,Wang Yi Kan,Zhang Allen,Lesurf Robert,Bashashati Ali,Funnell Tyler,Shiah Yu-Jia.Integrated single-nucleotide and structural variation signatures of DNA-repair deficient human cancers[EB/OL].(2025-03-28)[2025-05-08].https://www.biorxiv.org/content/10.1101/267500.点此复制

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