三链核酸介导ErbB-2交联的细胞功能调控

Epidermal growth factor receptor 2 (ErbB-2) is a key oncogenic biomarker overexpressed on the cell membranes of breast, gastric, colorectal, ovarian and bladder cancers. In addition, ErbB-2 often induces autodimerization or heterodimerization with other members of the ErbB family, leading to the activation of signal transduction pathways related to cancer cell survival and malignant phenotype, thereby promoting uncontrolled cell growth and inducing carcinogenesis. It is of great significance to develop new therapeutic methods targeting ErbB-2 receptor degradation. In this study, a three-stranded aptamer probe TrplexDNA binds to ErbB-2 receptors on cell membrane was designed to regulate the number of ErbB-2 receptors, enhance the ability of transporting ErbB-2 receptors to lysosomes for degradation, and destroy the homologous/heterologous dimer of ErbB-2. Inhibit the activation of downstream signaling pathways and inhibit the proliferation, growth and migration of tumor cells. TrplexDNA can also shorten the distance of ErbB-2 receptors on the cell membrane, resulting in receptor crosslinking effect, calcium influx and Caspase activation, and further enhance cell apoptosis.