四个miRNA在鸡性分化早期的性别差异表达谱分析
he sexually dimorphic expression profile analysis of four miRNAs in chicken embryo at the early stages of sex diferentiation
miRNA 是一类长度约为20～24 个核苷酸的小分子非编码RNA，它通过调控靶基因转录后的表达而在多种生物学途径中发挥作用。为探讨miRNA在性腺早期发育中的作用，本课题组在前期通过基因芯片方法筛选出了在E3.5～6.5 天雌、雄鸡胚中呈差异表达的若干个miRNAs，本研究即采用茎环状引物的定量PCR方法对其中4个miRNAs 进行表达谱分析。结果表明，miR-107、1767这2个miRNAs呈现前期雌性高表达后期雄性高表达的趋势，miR-2954的表达则是在E4.5d雄性中极显著高于雌性(P<0.01)，miR-92在所检测的各时期（除E4.5d外）中的表达均为雄性极显著高于雌性 (P<0.01)。本文根据表达谱结果推测了miR-92可能涉及早期鸡胚性腺发育过程。
miRNA is a family of small, non-coding RNA molecules of about 20-24 nucleotides, which regulate many biological pathways by down-regulate target gene expression at the post-transcriptional level. In order to investigate the function of miRNA on the early development of chicken gonad., some sexually dimorphic miRNA expressions during E3.5~6.5 in chicken embryos had been found by miRNA chip in previous studies. In this study, expression patterns of 4 miRNAs which selected based on the early study were studied by stem-loop Quantitative PCR methods. The results showed miR-107 and 1767 were higher expression in female during early stage, but higher in male during later stage; miR-2954 was with significant higher expression in male at E4.5d(P<0.01), and miR-92 was expressed at significant higher level in male at all detected stages, except for E4.5d (P<0.01). Based on the expression results, it is speculated that miR-92 should have effected on the early process of gonad development in chicken embryo.
王晓、苗楠、龚炎长、俸艳萍
分子生物学遗传学动物学
鸡miRNA性腺发育定量PCR
chickenmiRNAgonad developmentQuantitative PCR
王晓,苗楠,龚炎长,俸艳萍.四个miRNA在鸡性分化早期的性别差异表达谱分析[EB/OL].(2012-01-16)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201201-559.点此复制
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