低磷胁迫下杉木针叶蛋白的提取与表达差异分析
Variance analysis of protein extraction and expression forChinese fir needles under low phosphorus stress
本文采用TCA-丙酮沉淀高速离心法，同时对杉木针叶蛋白质组双向电泳技术中的全细胞蛋白的提取、蛋白的裂解、电泳条件等环节进行了优化改良，获得了高重复性、高清晰度的杉木针叶响应低磷胁迫的差异蛋白图谱。在此基础上对低磷处理5d的杉木进行针叶蛋白提取。并使用ImageMaster 2D Platinum 7软件分析比较杉木针叶在对照与低磷处理下的蛋白表达谱。研究表明图谱中有59蛋白点存在显著差异可能与低磷胁迫有关。其中，上调表达点52个，下调表达点7个。
In order to obtain the protein profile with high repeatability and resolution under the condition of phosphorus deficiency, the high-speed centrifugation of TCA-acetone precipitation and the optimized electrophoresis including the extraction of whole-cell protein in Chinese fir needles, the protein cleavage and the condition of electrophoresis were used in this study. The protein extraction of fir needles under 5d low-phosphorus treatment was conducted, and the comparison of protein profile between control and the treatment samples were made by using ImageMaster 2D Platinum 7 software. The results showed that there were 59 protein points of low phosphorus treatment significantly different from the control sample, which included 52 upregulated and 7 downregulated points.
何琳琳、刘爱琴、刘鑫鼎、赵中华、吴鹏飞
生物科学研究方法、生物科学研究技术生物化学植物学
生态学杉木针叶低磷胁迫双向电泳
Ecologyhinese firneedlesPhosphorus stressTwo-dimensional electrophoresis
何琳琳,刘爱琴,刘鑫鼎,赵中华,吴鹏飞.低磷胁迫下杉木针叶蛋白的提取与表达差异分析[EB/OL].(2014-05-19)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201405-309.点此复制
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