二妙丸中三种生物碱成分在beagle犬体内的药代动力学研究

o establish an LC-MS/MS method for the simultaneous determination and plasma pharmacokinetic study of berberine, jatrorrhizine and palmatine in beagle dogs after oral administration of Ermiao wan. Six dogs (3 males and 3 females) were included in the study with a single orally dose of Ermiao wan equivalent of 25mg/kg berberine. Blood samples were collected into heparinized tubes in predetermined time intervals from 0 to 48 hours. The plasma concentrations of berberine, jatrorrhizine and palmatine were determined by the LC-MS/MS. The pharmacokinetic parameters were calculated by DAS2.0 software. The main pharmacokinetic parameters of berberine, jatrorrhizine and palmatine were found as follows: Cmax (4.474±1.8), (0.5083±0.2) and (0.8124±0.7) ng/mL, t1/2 (38.1±15.4), (23.8±9.9) and (20.2±17.1) h，AUC(0-τ) (37.4±3.9), (3.75±1.2) and (3.01±0.9) ng h/mL. The established LC-MS/MS method was sensitive and accurate for the simultaneous determination and pharmacokinetic study of berberine, jatrorrhizine and palmatine after a single oral administration of Ermiao wan in beagle dogs. The results indicated that the proberberines were absorbed quickly and eliminated slowly after oral administration. These may provide useful information for clinical pharmacokinetic study.