北京麋鹿新孢子虫血清流行病学调查
SEROPREVALENCE OF ANTIBODIES AGAINST NEOSPORA CANINUM IN PèRE DAVID'S DEER (ELAPHURUS DAVIDIANUS) IN BEIJING
新孢子虫病是能够引起犬神经肌肉系统疾病和牛流产的一种机会性原虫病，广泛分布于世界各地，能感染多种野生动物。本研究利用免疫印迹法和间接荧光检测法，对北京地区49头麋鹿血清进行了新孢子虫抗体检测。结果显示，新孢子虫血清抗体阳性率为26.53%，且两种检测方法符合率良好。免疫印迹法可观察到阳性血清能够与新孢子虫全虫抗原在16、25和37kDa有明显的反应条带。这是我国首次关于麋鹿的新孢子虫感染情况的报道。结果提示新孢子虫病应该作为麋鹿流产的潜在因素加以考虑。
Neospora caninum is an apicomplexan protozoan that causes neuromuscular disease in canids and abortion in cattle worldwide. In China, sera from 49 Père David's deer (Elaphurus davidianus) were investigated for the presence of antibodies against N. caninum using indirect fluorescence antibody tests (IFAT) and western blots. N. caninum antibodies were found in 26.53% (13 positive/49 tested) of the Père David's deer according IFAT and the western blots. Western blot analysis revealed there was seroreactivity against immunodominant N. caninum antigens, and the bands were 16, 25, and 37 kDa in size together with other visible bands. This is the first study of antibodies against N. caninum in Père David's deer from China.
张昌盛、傅勇、刘晶、刘群、韩方洁
基础医学微生物学动物学
新孢子虫麋鹿血清流行病学
Neospora caninumPère David's deerSeroprevalence
张昌盛,傅勇,刘晶,刘群,韩方洁.北京麋鹿新孢子虫血清流行病学调查[EB/OL].(2015-07-07)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201507-56.点此复制
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