柱层析法制备高纯蛋黄磷脂酰胆碱
Preparation of high-purity egg yolk phosphatidylcholine by column chromatography
本文以蛋黄磷脂粗品为原料，在等梯度洗脱方式下，采用中性氧化铝柱层析对蛋黄磷脂酰胆碱的纯化进行研究。考察了氧化铝目数，乙醇浓度，洗脱流速和上载量等因素对磷脂酰胆碱含量、溶血磷脂酰胆碱含量以及磷脂酰胆碱的回收率的影响，确定了中性氧化铝柱层析纯化蛋黄磷脂酰胆碱的最佳工艺参数。结果表明：氧化铝目数为100~200，乙醇浓度95%，洗脱流速3mL/min，上载量0.0625g/(g固定相)，在上述工艺参数下，柱层析精制产品中磷脂酰胆碱的纯度为92.25%，溶血磷脂酰胆碱的含量为2.92%，磷脂酰胆碱的回收率为80.17%。
his research was made to purify egg yolk phosphatidylcholine by isocratic elution using neutral aluminum oxide column chromatography. According to the content of phosphatidylcholine(PC), lysophosphatidylcholine(LPC) and the recovery of phosphatidylcholine, this paper studied the effects of mesh of aluminum, concentraction of ethanol, flow rate and loading amount. The optimal technological parameters were obtained as follows: the mesh of aluminum oxide was 100~200; the concentration of ethanol was 95%; the flow rate was 3.0mL/min; the loading amount was 0.0625g/(g aluminum oxide). Under the condition, the product yield was 80.17%, phosphatidylcholine content was 92.25%, lysophosphatidylcholine content was 2.92%.
杨严俊、孙俊、苏宇杰、田莹
生物化学生物工程学
蛋黄卵磷脂磷脂酰胆碱溶血磷脂酰胆碱氧化铝柱层析
egg yolk lecithinphosphatidylcholinelysophosphatidylcholinealuminum oxidecolumn chromatography
杨严俊,孙俊,苏宇杰,田莹.柱层析法制备高纯蛋黄磷脂酰胆碱[EB/OL].(2011-10-02)[2025-08-10].http://www.paper.edu.cn/releasepaper/content/201110-8.点此复制
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