一株腈水合酶产生菌的驯化、保存与活化

he conditions of domestication, preservation and activation on a nitrile hydratase producing strain Rhodococcus sp.JSHM-1 were studied in this paper. The results show that domesticating the strain gradually using acrylonnitrile, when the concentration of acrylonnitrile accounted to 8 g/L, the result of domesticating was the most obvious, that was the enzyme activity raised from 476.8U to 962.4U. During the period of preservation, the enzyme activity of the strain was kept best when the concentration of acrylonnitrile was 4g/L, which was 927.5U. When activated the aged strain, the method of solid culture was better than fluid culture, and the enzyme activity under the solid culture method as adding 4g/L acrylonnitrile could increase to 971.6U although it was only 596.2U before activating.