中国HIV VPR基因多态性分析
iversity of the vpr gene of human immunodeficiency virus type 1 in South China
Little sequence information is available for human immunodeficiency virus type 1(HIV-1) vpr genes of Chinese origin. In our study, we analyzed HIV-1 vpr genes isolated from HIV-1 infected patients of South China. Reverse transcription polymerase chain reaction (RT-PCR) and nested PCR was used to amplify HIV-1 vpr gene. Using BLAST, Clustal W and Mega biological analysis software, we found vpr sequences mainly included subtypes as follows: CRF01-AE (51.63%), subtype C (24.84%), subtype B (17.65%), CRF03-AB (3.92%) and CRF08-BC (1.31%). The proportion of the glutamine (Q) is 84.3％ in the 77th position of the 154 deduced amino acid sequences, which differ from overseas reports.
Little sequence information is available for human immunodeficiency virus type 1(HIV-1) vpr genes of Chinese origin. In our study, we analyzed HIV-1 vpr genes isolated from HIV-1 infected patients of South China. Reverse transcription polymerase chain reaction (RT-PCR) and nested PCR was used to amplify HIV-1 vpr gene. Using BLAST, Clustal W and Mega biological analysis software, we found vpr sequences mainly included subtypes as follows: CRF01-AE (51.63%), subtype C (24.84%), subtype B (17.65%), CRF03-AB (3.92%) and CRF08-BC (1.31%). The proportion of the glutamine (Q) is 84.3％ in the 77th position of the 154 deduced amino acid sequences, which differ from overseas reports.
李慧、谌资、刘猛、周华英、张春迎、郑煜煌
基础医学生物科学研究方法、生物科学研究技术
多态性VPR基因艾滋病病毒
iversityvpr geneHIV
李慧,谌资,刘猛,周华英,张春迎,郑煜煌.中国HIV VPR基因多态性分析[EB/OL].(2010-03-31)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/201003-1093.点此复制
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