溶栓及抗凝药物对内皮细胞纤溶功能的影响

o study The effect of fibrinolytic or anticoagulant drugs on the fibrinolytic function of HPAECs. Methods When the HPAECs reached confluence, starvate for 12 h, and incubate the cells with different drugs for 12 h. Cells were classified into 5 group according to different drug: control group, thrombin(1 IU/ml) group; thrombin (1 IU/ml) and pro-urokinase (150 IU/ml) group, thrombin (1 IU/ml) and rt-PA (150 IU/ml) group and thrombin (1 IU/ml) and LMWH (10 IU/ml) group. After incubation, collect the medium, and detect the content of u-PAR, PAI-1 and TF. Results Compared to thromin group, the content of u-PAR in pro-urokinase group was statistically increased (0.51±0.04 VS 0.37±0.01); The content of PAI-1 in Pro-urokinase group and rt-PA group were statistically decreased（53.4025±3.09，44.1750±1.88 VS 107.9963±5.95）; The content of TF in rt-PA group and LMWH group were statistically decreased（116.6313±7.49，156.7563±11.07 VS 231.4063±20.42）. Conclusion Pro-urokinase, rt-PA and LMWH have direct and different effect on the fibrinolytic and anti-coagulant function of HPAECs, which may be taken into consider in different thromboembolic diseases.