壳聚糖包裹纳米金粒子高灵敏检测汞离子
Highly sensitive determination of Hg2+ by chitosan coated gold nanoparticles
汞离子由于具有很强的毒性,且来源十分广泛,因此人们对于它的微量检测技术一直都十分关注。为了实现汞离子的高灵敏度检测,将表面增强拉曼作为检测技术,制备出以对巯基苯甲酸为信号分子,壳聚糖为包覆材料的拉曼探针:当溶液中存在汞离子时,汞离子和壳聚糖会发生螯合作用,从而使纳米金团聚形成"热点",产生表面增强拉曼散射效应。通过检测对巯基苯甲酸的拉曼信号强度可绘制标准曲线达到高灵敏检测汞离子的目的。通过这种方法虽然特异性不尽如人意,但是灵敏度却是达到了预期结果,最终优化后汞离子分析测定的线性范围为40-200 nmol/L,最低检测浓度为40 nmol/L。
People have always been concerned about mercury ion trace detection due to it's highly toxic and a wide range of sources.Surface-enhanced Raman scattering was applied as new technology to detect mercury ion with high sensitivity.Raman probe was developed using chitosan as coating material and 4-MBA as Raman reporter:In the mercury ion solution,Chitosan coated gold nanoparticles would be aggregated due to the coordination of chitosan and mercury ions.Then the "hot spot" will form and surface-enhanced Raman scattering effect will appears.Purpose of highly sensitive detection of mercury ions can be achieved by detecting the signal strength of 4-MBA.Although the specificity of this approach is not satisfactory, the sensitivity has reached the requirements.After optimization, the detection range for mercury was 40-200 nM/L and the limit of detection is 40 nM/L.
沈爱国、裴金菊、刘翼振、胡继明、陈勇
生物化学生物物理学化学
表面增强拉曼散射壳聚糖纳米金汞离子
SERSChitosangold nanoparticlesHg2+
沈爱国,裴金菊,刘翼振,胡继明,陈勇.壳聚糖包裹纳米金粒子高灵敏检测汞离子[EB/OL].(2013-03-11)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201303-409.点此复制
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