茉莉酸甲酯对杠柳不定根中重要次生代谢产物诱导及生理机制的研究

Objective:To studyt on the inducing effect of methyl jasmonic acid (MeJA) on Periploca sepium Bunge adventitious roots in the secondary product Periplocin and 4 - methoxy salicylaldehyde biosynthesis, and discuss the physiological mechanism in the process of induction. Methods: Culture Periploca sepium Bunge adventitious roots and add MeJA with 10, 100, 200 mmol / L into the cultures at the stable stage (18-20 d). The cultures were induced for 3 days and then the adventitious roots were harvested and measured with physiological index and the content of periplocin and 4- methoxy salicylic aldehyde. Results: The contents of proline, soluble sugar, SOD, POD and CAT first increased and then decreased with the increaseing of the concentration of MeJA. The content of MDA increased and the content of solube protein reduced. The induced synthesis of periplocin and 4- methoxy salicylaldehyde was the strongest when the concentration of MeJA was 10 mmol/L (2.75 times and 2.26 times compared with control group,respectively). Conclusion: Low concentration of MeJA can effectively promoted the accumulation of periplocin and 4- methoxy salicylic aldehyde in Periploca sepium adventitious roots. MeJA, a kind of elicitor, can stimulate the plant's protection mechanism, clear free radicals and increase resistance of the culutres.