鸡胚心肌细胞分离培养研究

Isolation and characterization of chicken embryonic cardiomyocytes are critical for understanding on their biological properties. Cultured the chicken embryonic cardiomyocytes is one of key technologies to do some research about cardiac development, homeostasis and cardiovascular diseases. Here we described a method for the isolation and identification in vitro culture of chicken embryonic cardiomyocytes. Isolated cardiomyocytes were identified by cellular morphology and immunocytochemistry. The results showed that the cultured cardiomyocytes from the chicken embryos exhibited synchronous contraction and typical morphology, and stained positive for α- sarcomeric actin viewed under an inverted microscope. In conclusion, we provide a protocol for the reliable method of isolation and cultivation of chicken embryonic cardiomyocytes may allow more detailed analysis of their properties, and provide a valuable model for studying pathological processes including pulmonary hypertension, ascites and cardiovascular diseases.