Neto-α controls synapse organization and homeostasis at the Drosophila neuromuscular junction
Neto-α controls synapse organization and homeostasis at the Drosophila neuromuscular junction
Summary Glutamate receptor auxiliary proteins control receptor distribution and function, ultimately controlling synapse assembly, maturation and plasticity. At the Drosophila neuromuscular junction (NMJ), a synapse with both pre- and post-synaptic kainate-type glutamate receptors (KARs), we show that the auxiliary protein Neto evolved functionally distinct isoforms to modulate synapse development and homeostasis. Using genetics, cell biology and electrophysiology we demonstrate that Neto-α functions on both sides of the NMJ. In muscle, Neto-α limits the size of the postsynaptic receptors field. In motor neurons, Neto-α controls neurotransmitter release in a KAR-dependent manner. Furthermore, Neto-α is both required and sufficient for the presynaptic increase in neurotransmitter release in response to reduced postsynaptic sensitivity. This KAR-independent function of Neto-α is involved in activity-induced cytomatrix remodeling. We propose that Drosophila ensured NMJ functionality by acquiring two Neto isoforms with differential expression patterns and activities.
Nguyen Peter、Li Moyi、Hernandez Roberto X.、Vicidomini Rosario、Ramos Cathy Isaura、Macleod Gregory T.、Wang Qi、Serpe Mihaela、Stawarski Michal、Han Tae Hee、Jarnik Michal
Section on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human DevelopmentSection on Neuronal Connectivity, Eunice Kennedy Shiver National Institute of Child Health and Human Development,Department of Biology, Florida Atlantic UniversitySection on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human DevelopmentSection on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human Development||The Institute of Functional Genomics of LyonDepartment of Biology, Florida Atlantic UniversitySection on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human DevelopmentSection on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human DevelopmentDepartment of Biology, Florida Atlantic University||Biomedical Department, University of BaselSection on Cellular Communication, Eunice Kennedy Shiver National Institute of Child Health and Human DevelopmentCell Biology and Neurobiology Branch, Eunice Kennedy Shiver National Institute of Child Health and Human Development
细胞生物学遗传学分子生物学
Synapse developmentsynapse homeostasisionotropic glutamate receptorsauxiliary proteinsNetoDrosophila neuromuscular junction
Nguyen Peter,Li Moyi,Hernandez Roberto X.,Vicidomini Rosario,Ramos Cathy Isaura,Macleod Gregory T.,Wang Qi,Serpe Mihaela,Stawarski Michal,Han Tae Hee,Jarnik Michal.Neto-α controls synapse organization and homeostasis at the Drosophila neuromuscular junction[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/812040.点此复制
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