远志种质资源遗传多样性ISSR分析
Genetic diversity of Polygala tenuifolia Willd and P. sibirica L.
目的：用RAPD技术分析远志Polygala tenuifolia Willd.及卵叶远志P.sibirica L.的遗传多样性。方法：用CTAB法提取基因组DNA。反应总体积25µl，内含10×PCR buffer2.5µl、dNTPs 0.2mmol/L、MgCl22.0 mmol/L、引物0.2µmol/L、模板40 ng、Taq酶0.3µl。扩增程序为：94℃预变性5min，94℃变性0.30min,Tm复性0.45min,72℃延伸2min，循环40次：72℃延伸5min。结果：5条引物扩共增出96条DNA片段，其中有84条具多态性，平均多态性扩增产物为87.5%。不同的ISSR扩增引物，扩增片段的多态性因引物而异，多态性片段为3～8条，平均每条引物能扩增4条多态性带；距离系数为17时，11个样品聚为远志Polygala tenuifoliaWilld.居群和卵叶远志P.sibirica L.居群两类。结论：远志与卵叶远志具有丰富的遗传多样性，遗传聚类与其地理分布有一定的相关性。
Objective: To analyze genetic diversity of Polygala tenuifolia Willd and P. sibirica L. with ISSR-PCR. Methods: Genome DNA was extracted with CTAB method. The ISSR-PCR reaction system was constructed with 2.5µl 10×PCR buffer, 0.2mmol/L dNTPs, 2.0 mmol/L MgCl2, 0.2µmol/L primer, 40ng template and 0.3µl Taq with total reaction volume being 25µl. PCR amplifying sequences as follows: Initial denaturating for 5min at 94℃,then denaturating for 0.3min at 94℃, annealing for 0.15min and extension for 2.0min at 72℃. After 40 times cycle, keeping extension for 5min. Results : 96 polymorphism strands were detected with 5 primers, among which 84 strands were of polymorphism. Cluster analysis indicated that all samples can be clustered into two populations with distance coefficient being 17. Conclusion: Polygala tenuifolia Willd and P. sibirica L. have rich genetic diversities. The genetic clustering has some relevance with geographical distribution of P. tenuifolia Willd and P. sibirica L.
万德光、王光志
遗传学植物学分子生物学
远志卵叶远志遗传多样性RAPD
Polygala tenuifolia Willd.Polygala sibirica L.Genetic diversityISSRPCR
万德光,王光志.远志种质资源遗传多样性ISSR分析[EB/OL].(2008-07-28)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/200807-521.点此复制
评论