去分化间充质干细胞体内成骨再分化潜能的实验研究

Objective: To explore the osteogenic redifferentiation potential of de-differentiated MSCs (De-MSCs) in vivo. Methods: De-MSCs and MSCs were resuspended in osteogenic induction medium and seeded in collagen scaffolds with the same concentration. The scaffolds with cells were subcutaneously implanted into SCID mice. After implantation for 7 days, the collagen scaffolds were collected and ALP activity assay and RT-PCR assay were performed to detect the expression of osteogenic related genes. After implantation for 30 days, the collagen scaffolds were collected and HE staining and immunohistochemistry staining were performed. Results: The results of ALP activities and expression levels of osteogenic related genes (BMP2, Runx2, Osterix and DLX5) in De-MSCs group were obviously higher than those in MSCs group. The HE staining of De-MSCs group and MSCs group showed ectopic new bone formation. The results of immunohistochemistry staining showed the positive expression rate of collagen type II of osteoblast cell membrane in De-MSCs group was higher than that in MSCs group. Conclusions: Compared with MSCs, De-MSCs have higher osteogenic differentiation efficiency in vivo. De-MSCs could be an alternative potential seed cells in tissue engineering and regenerative medicine.