miR-223在肾透明细胞癌中的表达与功能
目的探讨miR-223在肾透明细胞癌中的表达与功能。方法用real-time RT-PCR法检测miR-223在肾透明细胞癌组织和瘤旁组织中的表达以及细胞系中miR-223的表达量。用transwell迁移实验和划痕实验测转染miR-223模拟物的786-O细胞的迁徙能力，MTS法测其增殖情况，流失细胞仪分析其细胞周期。并用SPSS统计软件分析其临床数据与miR-223表达量的关系。结果miR-223在肾透明细胞癌组织和细胞系中的表达量高于瘤旁组织和正常肾小管上皮细胞(P=0.0003 )，其miR-223的表达量与肿瘤的大小有关，肿瘤直径大于4 cm的肿瘤miR-223表达量升高(P=0,0028)。转染miR-223模拟物的786-O细胞transwell细胞迁移数比对照组多(P<0.0001)，划痕实验显示实验组愈合能力比对照组快，增殖实验表明实验组增殖速度快于对照组(P=0.006)，细胞周期显示实验组细胞G1期减少，S期细胞增加，提示处于增殖的细胞数多(P<0.05 )。结论miR-223可能起着促进肾透明细胞癌发生发展的作用，可能是抗肾透明细胞癌治疗的新的抗癌靶标。
Objective To investigate the expression of miR-223 in clear cell renal cell carcinoma (ccRcc) and its clinical implications. Methods Quantitative real-time PCR was employed to detect the levels of miR- 223 expression in ccRcc pair-matched adjacent normal tissues and different renal cancer cell lines. Transwell migration essay and wound healing essay were used to evaluate the invasion and migration of renal cancer 786-0 cells transfected with miR-223 mimics. MTT essay was used to measure the cell proliferation, and the cell cycle changes following the transfection were analyzed with flow cytometry. Results Compared with the normal tissues, the cancer samples showed up-regulated miR-223 expression, which was associated with tumor size. In 786-0 cell cultures, transfection with miR-223 mimics significantly enhanced cell migration (P<0.0001) and growth (P=0.006) and induced G1 cell cycle arrest. Conclusion miR-223 promotes renal cancer cell migration and proliferation and may serve as a potential therapeutic target for ccRcc.
牛少曦；马鑫；张瑜；巩会杰；高宇；李新涛；沈东来；王雷；姚远新；张旭
肿瘤学基础医学分子生物学
miR-223肾透明细胞癌迁移增殖
牛少曦；马鑫；张瑜；巩会杰；高宇；李新涛；沈东来；王雷；姚远新；张旭.miR-223在肾透明细胞癌中的表达与功能[EB/OL].(2017-12-07)[2025-08-17].https://chinaxiv.org/abs/201712.01140.点此复制
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