响应面法优化棕壤茶园专用解磷微生物菌剂制备工艺

Response surface methodology was used in order to optimize the preparation technology for Brown tea garden special phosphate solubilizing microorganism agent. Three key factors that affect the preparation technology for tea microbial agent were got by Plackett-Burman design, including ratio of water and carrier (X1)、fermentation temperature (X2) and dry temperature(X3). A Quadratic regression equation model was established according to steepest ascent experiment, central composite design and response surface methodology , which the response value was the effective number of living bacteria in agents. The model predicted that the maximum number of the effective living bacterium was 5.01×1010 and the optimal preparation conditions were as follow: ratio of water and carrier was 1.8, the amount of bacterial inoculum was 12.5% of carrier, the temperature of fermentation and drying were 30℃ and 41 ℃ respectively. The effective number of living bacteria in agents that prepared using the above optimization process conditions which basically consistent with the maximum predicted value by the model. These results suggested that the predicted model was reliable.?????