龙牙楤木皂苷IV对心肌细胞缺氧/复氧损伤的保护作用

Objective：To investigate protective effects of Tarasaponin IV on H9c2 cardiac myocytes exposed to simulated hypoxia- reoxygenation. Methods: This experiment Were divided into five groups: normal control group (group C); hypoxia / reoxygenation model group (H/R group); hypoxia / reoxygenation model + Tarasaponin IV low (12.5μg/ml), medium (25μg/ml), high (50μg/ml) dose group . Use of cardiac H9c2 cells cultured in vitro, using hypoxia / reoxygenation (H/R) method of myocardial cell injury induced H9c2 to simulate the clinical myocardial ischemia-reperfusion injury, observe Tarasaponin IV of the hypoxia protective effects of H9c2 myocardial cell injury. Tarasaponin IV intervention by MTT assay Cell viability; Hoechst33342/PI fluorescence staining Tarasaponin IV on hypoxia - reoxygenation-induced apoptosis of myocardial cells and JC-1 fluorescent probe assay H9c2 mitochondrial membrane potential.Results: Compared with the control group, H/R group cell viability was significantly decreased(P<0.01). Compared with the H/R group, cell viability and mitochondrial membrane potential was significantly increased(P<0.05) in Tarasaponin IV group, and significantly reduce myocardial cell apoptosis(P<0.05).Conclusion: Tarasaponin IV on myocardial hypoxia reoxygenation injury has a significant protective effect in a dose-dependent manner. The mechanism may be associated with decreased myocardial apoptosis, increased mitochondrial membrane potential.